BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 753

Peptides:

Sequences

RGDLKQLSELTW
RGDLAALSAPPV
SHARGDLVGLTP
ILARGDLSTLSA
NARGDLGTLPPR
RGDLPHQYQHPS
RGDLQYPRSLTL
RGDLMLPFWPRL
RGDLAAPHRTGA
RGDLNNYLLMML
RGDLPPVPMARE
RGDLPQERHYPR
RGDLLHASARAS
RGDAPSPNIFRL
VAPRGDRPTLPI
RGDYYANLANWL
HARGDFSWLMPE
RGDFAQLLITWQ
RGDIPSMSTLPR
RTDLDSLRTYTL
GDLDLLKLRLTR
GDLHSLRQLLSR
RTDLHTLSSTRH
RDDLHMLRLQLW
SSDLHALKKRYG
TSDLETLRLRLH
RDLETLYNRYAT
RHDLSELIRRTT
HPRTDLASLAKR
QRSDLASLKLTL
VTDLQSLYWRIK
RDDLQTLLNRLG
RSDLDTLLARHQ
QSGDLYTLRNSR
RSDLTALTQLLL
KGDLATLIRTNL
QSAHRGDIPNVL
ASDISALSARLR
RDFQYLWNRVSQ
VSDVYQLTERLR
AGDLVTFKLRLH
HHELDSLRARLM

Sequences Features:

Motif:

RGD, D-L-x(2)-L

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 9890954

Target Name:

Integrin alpha-V-beta-6, integrin αVβ6

Template Name:

Fibronectin, FN

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

RTDLDSLRTYTL, selected from the phage sequences was a selective inhibitor of RGD-dependent ligand binding to αvβ6 in isolated receptor assays (IC50 = 20 nM), and in cell adhesion assays (IC50 = 50 mM). DLXXL peptides were highly specific inhibitors of αvβ6-fibronectin interaction as synthetic scrambled or reversed DLXXL peptides were inactive.

Curator:

Curation Date:

0000-00-00

First |

Prev |

753

| Next

| Last