BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 744

Peptides:

Sequences	Affinity Values
PCQRAIFQSICN	[0.24]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11687659

Target Name:

Mosquito midgut lumen

Template Name:

Plasmodium

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

LX-8 phage display library (XCX8CX)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The absorbance at 450 nm was measured. Data shown were reproduced from the graph.

Brief Description:

For selection of phages that bind to the midgut lumen, phages (1e15/ml) suspended in 1 mg/ml of gamma globulin/120mM NaCl/20mM NaHCO3 were fed to the mosquitoes, and midguts were dissected 30 min later.

BiopanningDataSet Comments:

After the fourth round of selection, almost half of the phages from midguts displayed PCQRAIFQSICN. This peptide strongly inhibited Plasmodium invasion of salivary gland and midgut epithelia.

Curator:

Curation Date:

0000-00-00

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