BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 739

Peptides:

Sequences

Affinity Values

GPLGLS
GPLGLK
VRPLGI
APLGMS
PLSISG
PLQFRG
PLTMMG
PLPMRM
APLAMR
PLSIQD
PLSFQG
VLPLPM
PLSLLS
PAGLSD
PRGLVA
PKGLRA
PYGMRA
PVALKA
PKGVYS
PKGITS
PSGIHV
PFGFKS
GPPMSL
PINLHG
PHPLFL
PSELKG
MAPYAL
PFAFQG
PSAYHS
PMSYNA
PAEIVA
PMEMVE
VTPYNM
PPRAIR
PRPFNY

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Sequences Features:

Motif:

PLGMRG

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

2-5

Reference:

PMID: 10906330

Target Name:

Collagenase 3

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

fTC-LIB-N6 phage display library

Affinity Measurement Method:

Western blot

Affinity Measurement Description:

+++ strong cleavage; ++ some cleavage; + less cleavage; ND no cleavage of phage by human collagenase 3 detected by Western blotting. The selected phages were shown to be more sensitive by Western blot analysis to human collagenase 3 treatment than the positive control phage fTCol-3S (Western blotting result, [+]) with the sequences of PLGLWAR and the negative control phage fTCol-3R (NPVEPA) (Western blotting result, [ND]).

Brief Description:

BiopanningDataSet Comments:

Phage display screening also selected five substrate sequences that share sequence homology with a major MMP cleavage sequence in aggrecan and seven substrate sequences that share sequence homology with the primary collagenase cleavage site of human type II collagen. In addition, putative cleavage sites similar to the consensus sequence are found in human type IV collagen.

Curator:

Curation Date:

0000-00-00

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