BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 684

Peptides:

Sequences
SGHQLLLNKMPN(19) LWATFPPRPPWL(15) WSAAPTKPPYHT(5) ILANDLTAPGPR(4) HHGHSPTSPQVR(3) LPYGTSNRHAPV(3) YVQGWNYHDLTR(3) LWAAFPPQASVA(3) FDTPHTLTWFHG(2)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 15319030

Target Name:

Human glioma cell lines

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The library was screened against\r\na mixture of human glioma cell lines including dGli36, SF767, U87MG, U251MG, and U373MG at 37°C to increase the probability of obtaining sequences that could interact generically with most glioma cells. Phages rescued were further subjected to negative panning with nonglioma cell lines A549, CNE2, and\r\nHepG2, to eliminate nonspecific background binding.

BiopanningDataSet Comments:

LWATFPPRPPWL is able to target specifically to tumors of glial origin, which would allow the design of applications related to the diagnosis and treatment of human gliomas.

Curator:

Curation Date:

0000-00-00

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