BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 678

Peptides:

Sequences

GHLHLRVPTLKM
EFIRSPHSVDWL
SQSRNPPMPPPR
RRPPYRVPPKLF
SLYERHPASTYP
HTVSRRPLPSSG
RHTHGNLLRFPP
RKPTQSLPTRLV
TRRPHKMRSDPL
SRQFLHSLDRLP
QTPYQARLPAVA
RNNLNQTYPERR
YSLLPVRPVALT
HLHHHLDHRPHR
TLTWHTKTPVRP
WHPHRHHHLQWD

Sequences Features:

Motif:

R-x-P

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 16690883

Target Name:

Gap junction alpha-1 protein

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

After removing the unbound viruses by washing with Tris buffered saline containing 0.5% tween-20 (TBS-T), low affinity binders were eluted with a solution of TBS-containing Cx43CT at a concentration of 100 μg/ml. High affinity binders were then recovered by overlaying the well with a culture of E. coli, to allow the tightly bound phage to infect the bacteria.

BiopanningDataSet Comments:

The paper suggest that RXP-based peptides could serve as tools to help determine the role of Cx43 as a regulator of function in conditions such as ischemia-induced arrhythmias.

Curator:

Curation Date:

0000-00-00

First |

Prev |

678

| Next

| Last