BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 669

Peptides:

Sequences
FYSHSFHENWPS(26) IKSNPQTSNEFL(1) SAHGTSTGVPWP(1) ELANSVPWASPP(1) WSPGQQRLHNST(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

4-5

Reference:

PMID: 19891006

Target Name:

Troponin I, cardiac muscle

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

Thirty clones (20 from the 5th round and 10 from the 4th round) were sequenced. Kinetic indirect phage ELISAs revealed that FYSHSFHENWPS was found to have nanomolar affinities for for human troponin I while attached to the phage particles. However, the binding affinity was decreased by the presence of complex tissue culture media (MEM), and the addition of 10% calf serum further interfered with the binding of the target proteins. Coincidently, FYSHSFHENWPS was reported to be a BSA binder by SAROTUP.

Curator:

Curation Date:

0000-00-00

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