BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 655

Peptides:

Sequences
LTTAPKLPKVTR(1) QLGTQPNSRTYA(1) SRYITTMTNEQV(1) TVKHRPDALHPQ(1) LSTIGMPKLLA(1) VTSRTIIPQGSA(1) FAKSPDVSLNPS(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20067240

Target Name:

Human embryo carcinoma cell line NCCIT

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

phage display (biopanning and rapid analysis for selective interactive ligands, BRASIL panning, amplification-free panning)

BiopanningDataSet Comments:

From 370 clones tested, seven cell-binding phage clones were identified and sequenced. Of the seven phage-derived peptides identified, all supported cell binding except FAKSPDVSLNPS. The self-assembled monolayers presenting LTTAPKLPKVTR were especially effective, as they supported cell adhesion over the broadest range of densities.

Curator:

Curation Date:

0000-00-00

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