BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 636

Peptides:

Sequences
HWRTHHIHHFHQG MSPHHMHHSHGHG AKLAHHIHHFHGG WVPHHIHHFHRAG YTHHHHSWRLHTG AHHPHAWRHSHKG STFHFHTHKARHG HSQHHRFHHTYPG

Sequences Features:

Motif:

H(2)-[IM]-H(2)-F-H

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20090838

Target Name:

Serine-aspartate repeat-containing protein C

Template Name:

Neurexin-1-beta

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In fact, the target used in this study was N2 and N3 domain of SdrC (SdrCN2N3).

BiopanningDataSet Comments:

Fifty randomly picked plaques were selected and the corresponding phages were screened again for binding to SdrCN2N3, to SdrGN2N3 and to BSA as controls. All clones bound to SdrC, however, phage from eight clones displayed significantly higher binding to SdrCN2N3 compared to SdrGN2N3 and BSA. The clone inserts were sequenced.

Curator:

Curation Date:

0000-00-00

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