BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 613

Peptides:

Sequences
QQMHLMSYAPGP(19) AHRHPISFLSTL(12) VEAPLHRAQPHY(10) KMDRHDPSPALL(8) AYYPQNHKSKAE(2) APNHIPRPPGLT(1) YPHYSLPGSSTL(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21475935

Target Name:

NCI-H1299 non-small cell lung cancer cell line

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

NCI-H1299 cells were selected as the target cells, and the normal lung small airway epithelial cells (SAE cell line) were used as the absorber cells for a whole-cell subtractive screening.

BiopanningDataSet Comments:

After in vitro selection, 60 individual clones were chosen and sequenced. Seven of these clones were found to be lacking exogenous sequences. QQMHLMSYAPGP-bearing phages binded most effectively to NCI-H1299 cells. The synthetic peptide QQMHLMSYAPGP was demonstrated to be capable of binding to the tumor cell surfaces of NCI-H1299 and A549 cells and biopsy specimens, but not to normal lung tissue samples, other cancer cells, or non-tumor adjacent lung tissues.

Curator:

Curation Date:

0000-00-00

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