BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 610

Peptides:

Sequences
CTTQGNPQC(1) CATTKFSGC(1) CLSEQNRSC(1) CKLGFHGKC(1) CKTHAQHEC(1) CYEQHHPGC(1) CTTPHAWLC(1) CPMPRPSSC(1) CTDNTAKNC(1) CSLTTSTLC(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 19007831

Target Name:

Rat nose-to-brain

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The C7C phage peptide display library (Ph.D.-C7C) was applied intranasally to anesthetized rats and recovered phage from the brain tissue 45 min after phage administration. After three rounds of panning, 10 positive phage clones were selected and sequenced.

BiopanningDataSet Comments:

After nasal administration, Clone7 entered the brain within 30 min and exhibited translocation efficiency about 50-fold higher than a random phage. A 11-amino acid synthetic peptide derived from the displayed sequence of Clone7 (ACTTPHAWLCG, the flanking A and G were derived from the M13 coat protein) efficiently inhibited the nasal-brain translocation of Clone7. Both phage recovery results and fluorescent microscopy images revealed the presence of many more Clone7 phage in the brain than in the liver, kidney and other internal organs after the nasal administration, suggesting that Clone7 bypassed the BBB and entered brain directly. Furthermore, both Clone7 and the ACTTPHAWLCG peptide were found to be heavily distributed along the olfactory nerve after the nasal administration, further suggesting a direct passage route into the brain via the olfactory region.

Curator:

Curation Date:

0000-00-00

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