BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 600

Peptides:

Sequences
VRKRSECLGAHD(19)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 19778796

Target Name:

Hepatocellular carcinoma cell line HepG2

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Subtractive selection with human liver cell line L02 cells was performed.

BiopanningDataSet Comments:

Among 30 clones picked, 27 clones have proper insetions. However, only the most frequent sequence is given in the original paper. Immunocytochemistry and immunohistochemistry confirmed the specificity of the VRKRSECLGAHD bearing phage binding to the hepatoma cells.

Curator:

Curation Date:

0000-00-00

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