BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 591

Peptides:

Sequences
EGEVGLG(0.58) MRRSVGS(0.14) SSAVL(0.08) VLI(0.08) SAGSVAL(0.06) FGVR(0.03) GFWEGGL(0.03)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 19825959

Target Name:

Human breast cancer cell line MDA-MB-231

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X9 T7 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Mice bearing MDA-MB-231 human breast tumors were subject to 40 mg/kg sunitinib given i.p. for 3 consecutive d. Treatment began 30 d after tumor implantation. The phage libraries were administered 4 h after the last treatment. Phages were recovered after being in circulation for 16h by harvesting the tumors in the mice. Phages recovered from excised tumors were amplified and subjected to three more rounds of selection.

BiopanningDataSet Comments:

Thirty-six phage plaques were sequenced after the 4th round of panning. EGEVGLG was the dominant sequence isolated from biopanning. This peptide showed increased binding relative to control groups in two cancer cell lines (MDA-MB-435 and MCF-7 human breast) responding to sunitinib treatment, whereas no elevated binding occurred in vitro when samples were incubated with tumor cells that are unresponsive to sunitinib treatment (B16 melanoma and BxPC3 pancreatic).

Curator:

Curation Date:

0000-00-00

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