BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 587

Peptides:

Sequences
LVPPSFS(2) LPLTALP(2) LPLTPLP(2) HPVHHYQ(2) LPGIMSL(1) HKVVAYY(1) HSNTGYP(1) TIGLITS(1) TSGLASR(1) FPLLNML(1) NNLLPPY(1) SIVRLQV(1) SISVIQE(1) QPKQFFQ(1) AQCLRIP(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 19645415

Target Name:

Hp-AS-pwk RNA

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Preselection was conducted with prewashed beads to remove streptavidin-binding phage. For screening, the hp-AS-pwkRNA was refolded and hybridized with the 50-biotin-DNA in a 1:1 molar ratio. \r\nThe phages were eluted nonspecifically with 100 μL of buffer B (0.2M glycine-HCl, pH2.2, 1mg/mL BSA) for exactly 9 min followed by neutralization with 15 μL of buffer C (1M Tris-HCl, pH 9.1).

BiopanningDataSet Comments:

HPVHHYQ and LPLTPLP displayed selective binding to the A-site 16S rRNA with on-bead fluorescence assays.

Curator:

Curation Date:

0000-00-00

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