BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 578

Peptides:

Sequences	Affinity Values
SVSVGMLPSPRP(20) SSWILSPYHWGR(13) GSFASLTNPRVL(8) TIQHQNPPHYAV(7) SNPHTDNHWPGR(2)	[24.07 ± 3.40] [34.59 ± 8.20] [15.13 ± 1.36] [2.76 ± 0.20] [16.18 ± 1.59]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 19389430

Target Name:

Phytophthora capsici extract

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The apparent dissociation constant (Kd, pM) was obtained from a binding saturation curve fitted from three independent experiments.

Brief Description:

BiopanningDataSet Comments:

After five rounds of biopanning, 50 plaques were selected and sequenced. All the phages showed high binding affinity for P. capsici in the picomolar range. Phages bearing SVSVGMLPSPRP, TIQHQNPPHYAV and GSFASLTNPRVL inserts showed high specificity toward P. capsici, whereas GSFASLTNPRVL and SNPHTDNHWPGR bearing phages also bound to some of the similar Phytophthora strains with comparable affinities.

Curator:

Curation Date:

0000-00-00

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