BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 569

Peptides:

Sequences
FLLEPHLMDTSM(47) FLLEPHTVTWGA(16) FLIEPWHSSLQS(11) AFLFSPLLAWPT(6) TYRFGPLEPVAF(2)

Sequences Features:

Motif:

F-L-[LI]-E-P

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18563328

Target Name:

Hepatocellular carcinoma cell line HepG2

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In the process of the second round of panning, the amplified phages were incubated with human liver cell line L02 cells at first to remove phages binding to human liver cells.

BiopanningDataSet Comments:

Among 100 phages that specifically bound to and internalized in HepG2 cells, 82 clones demonstrated highly specific affinity to HepG2 cells and their binding to L02 cells was relatively weak.

Curator:

Curation Date:

0000-00-00

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