BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 524

Peptides:

Sequences

YTRISNDHHCDTTICHINST(3)
NDSNCTDTLCYVYPLHSNPG(2)
VKDALHHVDHTCSTFLCALP(2)
DTHFCDDNYCPLPHSSGAIA(2)
SCNDYVCLTPHPKTPLYEKQ(1)
NVNCNASVCTIPDRLITDNP(1)
SSYCNNSLCLVPSYPDHSNT(1)
HCNAHLCNLPDGSPSSISSH(1)
NIHHCNHIYCTIPSHS(1)
IHSYSYASDNMCTDTVCFIH(1)
CSDTYCTIPTYGRDRDVRRD(1)
DSYFCDNFLCRLDHNRNSRN(1)
HNISCDKSLCMLRESAPTTV(1)
NSSGSCDHFLCIIPTSNTHT(1)
HYSSASHANNVCDAHYCLLP(1)
AFCDISFCHLNDRIIIIITN(1)
GLDPYHRFCDDNVCSIRPFN(1)
DSSDDRHTCGHHICHLYPFS(1)
HPPSHYCDIHSRLCHPNAPD(1)

Sequences Features:

Motif:

C-[DNSTG]-D-[ST]-x-C-x(2)-P

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18672101

Target Name:

Thioredoxin-1, Trx-1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

ANL 22 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Biotinylated thioredoxin-1 imobilized with streptavidin-coated magnetic beads were used as target.

BiopanningDataSet Comments:

After three rounds of affinity selection, 96 phage clones were screened using a phage enzyme-linked immunosorbent assay (ELISA). A total of 24 phage clones bound specifically to E. coli Trx.

Curator:

Curation Date:

0000-00-00

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