BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 499

Peptides:

Sequences
NSLSNASEFRAP(6)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18330476

Target Name:

Angiopoietin-1 receptor

Template Name:

Angiopoietin-1, ANG-1

Structure of Target-Template Complex:

4K0V

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Phages bound to Tie2 receptors were eluted with angiopoietin-2.

BiopanningDataSet Comments:

After five rounds of screening, 20 phage clones were picked out randomly and amplified for DNA sequencing. Of these, only 17 clones had efficiently inserted peptides, and approximately 35% (6/17) of recovered clones expressed the consensus amino acid sequence NSLSNASEFRAP. Binding assays and Scatchard analysis revealed that NSLSNASEFRAP could specifically bind to Tie2 with a dissociation constant of 2.1×10−8 M. In addition, it wa showed that NSLSNASEFRAP was internalized into tumor cells highly expressing Tie2. Another enriched phage clone displayed a peptide XXGTHGHCQLSH, which did not have binding specificity for Tie2.

Curator:

Curation Date:

0000-00-00

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