BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 476

Peptides:

Sequences
NMIESMLRTASH(1) NPVEWFMSTVNT(1) NPIESMLRTASH(1)

Sequences Features:

Motif:

NPVE

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18715645

Target Name:

FVIII inhibitor positive plasma samples before rituximab treatment

Template Name:

Coagulation factor VIII

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The FVIII inhibitor titer of the target is 23 BUs/ml ( BU = Bethesda Unit, corresponds to 50% reduction of FVIII activity). An IgG preparation mixed 1:1 with plasma of a healthy donor was used in 2 negative selections to remove non-specific phages. For 3 rounds of positive selection, binding phages were eluted by competition with FVIII in excess. Although 300 phage clones were analyzed, only above sequences were given in the original paper.

BiopanningDataSet Comments:

The template of these peptides might be A2 domain of factor VIII.

Curator:

Curation Date:

0000-00-00

First |

Prev |

476

| Next

| Last