BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 474

Peptides:

Sequences
NPIEALM(1) NPVETQV(1) NPVEMLL(1) NPVEHMM(1) NPVESLL(1)

Sequences Features:

Motif:

NPVE

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18715645

Target Name:

FVIII inhibitor positive plasma samples before rituximab treatment

Template Name:

Coagulation factor VIII

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The FVIII inhibitor titer of the target is 23 BUs/ml ( BU = Bethesda Unit, corresponds to 50% reduction of FVIII activity). An IgG preparation mixed 1:1 with plasma of a healthy donor was used in 2 negative selections to remove non-specific phages. For 3 rounds of positive selection, binding phages were eluted by pH-shift using glycine-HCl, pH 2.2. Although 300 phage clones were analyzed, only above sequences were given in the original paper.

BiopanningDataSet Comments:

The template of these peptides might be A2 domain of factor VIII.

Curator:

Curation Date:

0000-00-00

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