BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 461

Peptides:

Sequences
CTLPHLKMC(10) CLYGNSDKC(3) CQHNHSKQC(3) CTKQHTSQC(3) CNPSLALHC(3) CSTGAHTQC(3) CDWTKPQSC(2) CTVKNGTLC(1) CQEGKMRLC(1) CSEHKTPMC(1) CQKQGHPSC(1) CTSTPFRIC(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18442789

Target Name:

Human B cell Burkitt lymphoma cell line Raji

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

MALDI-TOF mass spectrometry following immunoprecipitation assay showed that a potential template for the peptide CTLPHLKMC is a variable region of human immunoglobulin heavy chain which would be a specific phenotypic marker of Raji.

BiopanningDataSet Comments:

The phage clone encoding CTLPHLKMC peptide sequence avidly bound to Raji cells compared with control phage clones. Furthermore, flow-cytometric analysis on the biotinylated synthetic CTLPHLKMC peptide demonstrated the high binding affinity to Raji cells in a dose-dependent manner whereas it has binding activity to neither human peripheral bloodmononuclear cells including normal B cell derived from healthy donors nor other leukemia cells including THP-1, HL-60, Jurkat and IM-9.

Curator:

Curation Date:

0000-00-00

First |

Prev |

461

| Next

| Last