BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 457

Peptides:

Sequences
CWPGWDLLC(6) CWPGWDLNC(1) CWPGWDEMC(1) CWPGWDMAC(1) CSERPSQQC(1)

Sequences Features:

Motif:

C-W-P-G-W-x(2)-C

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 17673295

Target Name:

Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a

Template Name:

Fc domain of IgG (IGHG1,IGHG2,IGHG3,IGHG4)

Structure of Target-Template Complex:

3RY6

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In fact, the recombinant fusion protein HSA-FcγRIIa was used as the target. However, HSA-binding phages were removed using human serum albumin. For each round, panning parameters were wrote as (target) {wash} [elution]. Round 1, (100μg/ml HSA–FcγRIIa){nil}[0.1 mg/ml IgG]; round 2,(5μg/ml HSA–FcγRIIa){0.1 mg/ml IgG}[1mM DTT]; round 3,(5μg/ml HSA–FcγRIIa){0.1 mg/ml IgG}[1mM DTT].

BiopanningDataSet Comments:

The strongest binding clone CWPGWDLNC competed with IgG for binding to FcγRIIa and was inhibited from binding to FcγRIIa by the FcγRIIa-blocking antibody, suggesting that CWPGWDLNC and IgG share related binding sites on FcγRIIa.

Curator:

Curation Date:

0000-00-00

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