BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 443

Peptides:

Sequences
CPTWLSPAC(2) CPFSPSLKC(2) CSPTKSNSC(2) CQTTRSPIC(1) CQHASPTNC(1) CQPTPRSTC(1) CPTPNHDHC(1) CPTSHRNSC(1) CPTTKLSTC(1) CSIHGPTRC(1)

Sequences Features:

Motif:

SPT

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18569284

Target Name:

Colon cancer cells

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Laser capture microdissection (LCM) is performed to separate only cancer cells as the target used.

BiopanningDataSet Comments:

Thirty-seven clones were sequenced. Samples of tumor and noncancerous colon tissue were obtained at the time of colectomy from patients with operable colon cancer. Laser capture microdissection (LCM) is performed to separate only cancer cells as the target used. Initially, a subtraction step using non-cancerous colon tissue was performed. Phage clones displaying the SPT motif demonstrated 9-fold higher binding to colon cancer cells derived from a patient than insertless phage, while, recovery of the SPT phage from the colon cancer cell lines DLD-1 and HCT-15 was 7-fold higher than that of the control insertless phage. The binding of SPT phage to colon cancer cells from the patient was confirmed by immunofluorescence. Additionally, peptide SPTKSNS showed binding activity in the absence of mitogenic effects on colon cancer cells in vitro.

Curator:

Curation Date:

0000-00-00

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