BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 431

Peptides:

Sequences
VVFSTSV ISARSSP GDSMQQQ LQVHTQN WARSESP AALVRHT VRPMPLQ NQPPDSY APPWIAV LPSSYPP YTPLLPS

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18345013

Target Name:

Fresh human colonic adenomas

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Intact colon polyps freshly resected were used as the target.

BiopanningDataSet Comments:

The binding of VRPMPLQ to HT-29 human adenocarcinoma derived cells was around 20-fold greater than its binding to Hs738.st/int nonmalignant human intestinal cells. The bindings of APPWIAV, LPSSYPP and YTPLLPS to HT-29 were similar to wild-type insertless M13 phages. The fluorescein-conjugated VRPMPLQ bound more strongly to dysplastic colonocytes than to adjacent normal cells with 81% sensitivity and 82% specificity.

Curator:

Curation Date:

0000-00-00

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