BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 429

Peptides:

Sequences

CTTHISQTC
CHPSKALQC
CERLNLPQC
CTDNALKAC
CYKMDNHTC
CHVLDPHLC
CSRNHILTC
CVMSKHQHC
CLMGSSHSC
CPSHYTQAC
CHADQLPMC
CLPTPHHVC
CQLSLAPYC
CTFHSPRFC
CYATTLGAC
CGPSLRGVC
CFNTHTANC
CPGHHLSHC
CNSPKGKPC
CQWPGQSGC
CNSSSPTAC
CHQLMQNLC
CQATTARNC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18391034

Target Name:

Sin Nombre virus (SNV) strain SN77734

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

SNV was propagated and the titer was determined in Vero E6 cells. The preparation of UV-inactivated SNV was used for panning.

BiopanningDataSet Comments:

The phages were eluted with polyclonal anti-Gn-BSA IgG. They were obtained sera from a rabbit immunized with the bovine serum albumin (BSA)-conjugated peptide LKIESSCNFDLHVPATTTQKYNQVDWTKKSS, which corresponds to residues 58 to 88 of Gn from SNV strain SN77734. In all cases, the isolated peptides were less effective at blocking infection than were the same peptides presented by phage. Sin Nombre virus infection involves the interaction between viral membrane surface glycoproteins and the human integrin αvβ3. Some peptides selected are similar to human integrin beta-3 (CD61: P05106).

Curator:

Curation Date:

0000-00-00

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