BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 419

Peptides:

Sequences
CGPSEPIGAWDC CGPAEPRGAWVC CGPREPDGSWHC CGPVEPVGAWVC CGPWEPSGIWSC CGPVEPKGVWFC CGPYEPRGDWTC

Sequences Features:

Motif:

G-P-x-E-P-x(3)-W

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18184085

Target Name:

Anti-CD4 monoclonal antibody 5145A

Template Name:

T-cell surface glycoprotein CD4

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CL10 phage display library (CX10C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The human monoclonal antibody 5145A against HIV-1 gp120 was isolated from an asymptomatic, seropositive hemophiliac. The epitope of this HuMAb overlaps the CD4-binding site of gp120. The antisera from mimotope fusion protein immunized rabbit did not compete with CD4 for binding to gp120, although 5145A is specific for the conserved CD4 binding site region of the HIV envelope protein gp120. Perhaps, the template is not CD4.

BiopanningDataSet Comments:

GPYEPRGDWT and GPAEPRGAWV bearing phage clones had the highest apparent affinity, whereas GPSEPIGAWD-phage 2 had the lowest. Rabbits immunized with peptide-protein fusions produced antisera that bound to recombinant HIV-1 gp120, but did not bind to HIV-infected cells nor neutralize HIV. The antisera also did not compete with CD4 or antibodies to the CD4 binding site for binding to gp120.

Curator:

Curation Date:

0000-00-00

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