BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 410

Peptides:

Sequences
CRGDNFQC(9) CDVFTYWMRRDC(9) CDVFEVWMGRVC(4) CRGTQNTMRARC(1)

Sequences Features:

Motif:

D-V-F-x(2)-W-M-x-R-x

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18200546

Target Name:

Glioma cell line 9L

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CL6 phage display library (CX6C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Rat plasma was used as adsorbent before the first round. Before the the second and each subsequent round of panning, phages were preadsorbed on rat blood cells.

BiopanningDataSet Comments:

After the fourth and fifth panning rounds, 15 randomly selected phage clones were propagated and sequenced.

Curator:

Curation Date:

0000-00-00

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