BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 376

Peptides:

Sequences
CSNRDARRC CANKDVRRC CANLDTRRC CPNQDSRRC CPNGDERNC CVNNDGRLC

Sequences Features:

Motif:

C-x-N-x-D-x-R(2)-C

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

2-4

Reference:

PMID: 17259343

Target Name:

HT-1376 human bladder tumor cell line

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX7C T7 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The tumor cells were isolated from tumor xenografts in nude mice prepared using the HT-1376 human bladder tumor cell line. Phage that bound to normal bladder cells isolated from mouse bladder tissues, normal rat kidney cells, or human umbilical vascular endothelial cells were subtracted during the biopanning procedure.

BiopanningDataSet Comments:

After the second round of screening, the numbers of phage were enriched approximately by 1,000-fold and did not increase in further steps. Ninety-six phage clones were picked from the enriched phage library after the second and third rounds of screening. Twenty of the 96 clones showed selective binding to HT-1376 cells. These clones were sequenced. Six sequences were given in the published paper. It has been shown that CSNRDARRC selectively targeted and bound to primary bladder tumor tissues and can be used to detect tumor cells in urine.

Curator:

Curation Date:

0000-00-00

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