BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3681

Peptides:

Sequences

Affinity Values

TGSAKFLQRDTH(3)
AFADGYSARRNL(1)
AFKPTSGLAKLS(1)
AWRPFPSATSGP(1)
FAPYNNLSDNYP(1)
YHGQISANAHGW(1)
YSSIAPSISNAL(1)
YSSTSYRALTLG(1)
YTSLPTEATDRT(1)

[0.3523±0.0097]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 36746976

Target Name:

Vitamin D binding protein, VDBP

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Data are expressed as the means of the absorbance value at (410 ± SD) nm.

Brief Description:

After positive biopanning against the target, all 1.15 mL of neutralized phages were treated in a petri dish (polystyrene), and the solution was rocked at room temperature (RT) for 30 min. Only the supernatant from which the phage strongly bound to polystyrene was removed was collected.

BiopanningDataSet Comments:

Curator:

Bowen Li

Curation Date:

2023-10-13

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