BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3653

Peptides:

Sequences

Affinity Values

QQGWFPG
QQGWVLP
QGQAYVL
GQQGWGA
GGQFQWV
QGWHFGP
HQYGFGL
QWHLFAG

[8.7759±2.1469]
[7.9564±1.3562]
[5.1631±1.3274]
[4.8457±1.1427]
[4.055±1.1485]
[3.8415±0.8945]
[3.5587±1.3909]
[2.8777±0.9984]

Sequences Features:

Motif:

QQGWF

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 33545815

Target Name:

CD44/FC chimera

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Binding assay

Affinity Measurement Description:

The amount of phage in the solution was determined by titer count analysis. Binding amounts are relative to that of the original phage library. The relative binding amount of bound phages was reproduced from Figure 1a and shown.

Brief Description:

BiopanningDataSet Comments:

These clones contained the same 4 amino acid sequence, QQGW. We concluded that this 4 amino acid sequence is important for CD44 binding.

Curator:

Bowen Li

Curation Date:

2023-10-13

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