BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3643

Peptides:

Sequences

TLMVPRTGS(6)
WCQVQSVCA(3)
RWRRKALPW(2)
GWRPKMVLR(2)
RWRRKVLRH(2)
LSSCFRFCS(1)
WNMKFVWRS(1)
RPRARVFRF(1)
VWREPLHHH(1)
ARGPRRGVR(1)
RRVLSACVW(1)
VTLRFNGWG(1)
APAARNLEL(1)
VLVPVGVVW(1)
GAVSSHLDL(1)
GISGVSGCS(1)
LVYLVWDVW(1)
TWRRRLLVH(1)
TWRRRNLVH(1)
TWRKRMRLL(1)
GWRRKALMR(1)
GWRRKVWLR(1)
FWRRKVWHH(1)
RWRRKVFLH(1)
GWRRKVMRL(1)
FWRRKVLHH(1)
SWRRKIYHL(1)
GWRRKLRVV(1)
GWRRKSLWA(1)
GWRKKSLRL(1)
RWRKKAWHH(1)
RWKRKVLEA(1)
RWKRKVRVV(1)
MWKRKVLVL(1)
GWKRKTLSL(1)
RWKRKSLGR(1)
GWKRKYRQV(1)
TWKRKWFHH(1)
KYKRKRLFL(1)
RWKKKSWLW(1)
GRYRKNIMH(1)
GWFRKMLFS(1)
GWRPKTWIG(1)
GWKPKWLLD(1)
RWRAKVLPV(1)
GWRVKSIPV(1)
RWRVKSRKW(1)
GWRTKVGVL(1)
RWRPRVLPH(1)
RWRPRNWRH(1)
GWRARTLRV(1)
RWRARVRRL(1)
GWRARVLSL(1)
GWRTRVLPL(1)
RWRSRVLRI(1)
RWRMRALFH(1)
GWRPKRGYL(1)
SWRPKRLPW(1)
GWRAKRVIW(1)
GWRAKRMMV(1)
GWKAKRLSV(1)
RWRVKRYSH(1)
GWRVKRLVV(1)
GWRLKRLVD(1)
RWRVKRAML(1)
GWRSKRLRV(1)
GFRMKRLVV(1)
GWRFKRLEL(1)
GWRAKRRII(1)
AWRPRRLVV(1)
GWRARRLVI(1)
GWRVRRLFT(1)
GWKARKLVF(1)
GWLAKRAHH(1)
GWASKRLQV(1)
RWFSKRLLH(1)
VPRLKILVM(1)
ASRLKVHHH(1)
VVWGKMLSH(1)
WGWLRFEHH(1)

Sequences Features:

Motif:

PGGWRRKALSV and PGGWRAKVLSV

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 34919980

Target Name:

Cathepsin G-like

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X9 T7 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

A preference for a lysine in the P1 position of a substrate, arginines in positions P2 and P3, and the aromatic amino acid tryptophane in the P4 position was observed. Based on the sequence alignment we could identify a consensus sequence for this protease as being PGGWRRK↓ALSV. Mass spectrometry analysis of a peptide with the consensus sequence obtained by phage display showed that cleavage of this peptide occurred after the conserved Lys (K) residue.

Curator:

Bowen Li

Curation Date:

2023-10-13

First |

Prev |

3643

| Next

| Last