BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3639

Peptides:

Sequences	Affinity Values
SGVYKVAYDWQH HYSWSWIAYSPG	[1.9855±0.0209] [3.0371±0.0139]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

ABI PRISM 3100-Advanced Genetic Analyzer

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 34635758

Target Name:

GTTYGVCSK-biotin and Domain III of the envelope (E) glycoprotein (DIII)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Optical density data (450 nm) for each clone is expressed as the mean ± standard deviation (SD). Data shown were reproduced from Figure 1B.

Brief Description:

The first round of panning: target: GTTYGVCSK-biotin (1 μg/well), phage elution: competitive elution with GTTYGVCSK-biotin (9 μg) in PBS (pH 7.2). The second round of panning: target: GTTYGVCSK-biotin (1 μg/well), phage elution: competitive elution with GTTYGVCSK-biotin (2 μg) in PBS (pH 7.2). The third round of panning: target: GTTYGVCSK-biotin (1 μg/well), phage elution: non-related peptide in CovaBuffer (pH 7.2) + 1 M glycine; followed by (ii) competitive elution with GTTYGVCSK-biotin (2 μg) in PBS (pH 7.2). The 4th round of panning: target: rDIII (0.3 μg/well), phage elution: 0.1% TBST (pH7.2) + 250 mM imidazole. The 5th round of panning: target: rDIII (0.3 μg/well), phage elution: competitive elution with GTTYGVCSK-biotin (2 μg) in PBS (pH 7.2).

BiopanningDataSet Comments:

peptides, 4 cyclic peptides showed significant blocking of the interaction between DIII and hBMECs, and ability to neutralize infection in cultured cells.

Curator:

Bowen Li

Curation Date:

2023-10-13

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