BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3637

Peptides:

Sequences	Affinity Values
CVPKRRNNC CLKRQRT CRRRVS CNRRRA CRKRS CRKIR	[0.4316±0.0255] [0.3551±0.0538] [0.3453±0.0353] [0.5789±0.0212] [0.4203±0.027] [0.3482±0.0324]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

4-5

Reference:

PMID: 35014500

Target Name:

Kidney injury molecule 1, KIM-1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Byungheon Lee (Department of Biochemistry and Cell Biology, School Medicine, Kyungpook National University, Republic of Korea)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Optical density data (450 nm) for each clone is expressed as the mean ± standard deviation (SD) of three independent experiments. Data shown were reproduced from Figure 1.

Brief Description:

Each biopanning round consisted of subtraction of phages nonspecifically bound to M-270 epoxy beads only and subsequent selection of phages bound to KIM-1 conjugated epoxy beads with 3 M ammonium sulfate, followed by amplification of the eluted phages.

BiopanningDataSet Comments:

This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of kidney injury molecule-1 (KIM-1) expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model.

Curator:

Bowen Li

Curation Date:

2023-10-13

First |

Prev |

3637

| Next

| Last