BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3629

Peptides:

Sequences

AGKCCFATGGS(5)
ARKCCYGRGGS(4)
ASRKCCYVGGS(2)
AGGKCCYSGGS(1)
AGKCCYSKGGS(1)
AGKCCYSTGGS(1)
AKCCYAGRGGS(1)
AKCCYAHIGGS(1)
ANRKCCFAGGS(1)
ARAKCCYYGGS(1)
ASKCCYHVGGS(1)
AYKCCYWTGGS(1)
ASSFLEQDGGS(1)
AILIPFWTGGS(1)
AIPYCMGVGGS(1)
AWLRSMKVGGS(1)
ANTGWQSRGGS(1)
AVFFGVGWGGS(1)
AKKVWIIMGGS(1)
AMMTSSGGGGS(1)
AYFVVRGLGGS(1)

Sequences Features:

Motif:

KCC[FY]

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3-4

Reference:

PMID: 35665749

Target Name:

Zc RNase P ribozyme (RPR)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

AXXXXXXXGGS phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

We report that a hydrophobic-cationic RNA binding peptide selected by phage display (P43: AKKVWIIMGGS) forms insoluble amyloid-containing aggregates, which reversibly accrete RNA on their surfaces in an RNA-length and Mg2+- concentration dependent manner. The aggregates formed by P43 or its sequence-simplified version (K2V6: KKVVVVVV) inhibited RNA polymerase ribozyme (RPR) activity at 25mM MgCl2, while enhancing it significantly at 400mM MgCl2. Our work shows that such hydrophobic-cationic peptide aggregates can reversibly concentrate RNA and enhance the RPR activity, and suggests that they could have aided the emergence and evolution of longer and functional RNAs in the fluctuating environments of the prebiotic earth.

Curator:

Bowen Li

Curation Date:

2023-10-13

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