BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3611

Peptides:

Sequences
DHAQRYGAGHSG(20/30)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 36177466

Target Name:

Recombinant receptor-binding domain (RBD) of glycoprotein protein

Template Name:

Angiotensin-converting enzyme 2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Optical absorbance was measured at 450 nm in a microplate reader (Synergy H1, BioTek Instruments, Inc.,Winooski, VT, United States). Data shown were preproduced from Figure 1.

Brief Description:

BiopanningDataSet Comments:

In the competitive S-trimer-ACE2-binding experiments, synthetic C2 (DHAQRYGAGHSG) peptide inhibited S-trimer binding onto 293T-ACE2hR cells at high concentrations (50 mM) but not at lower concentrations (10 mM and below), neither for the settings of S-trimer binding onto recombinant ACE2 proteins.

Curator:

Bowen Li

Curation Date:

2023-10-13

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