BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3603

Peptides:

Sequences	Affinity Values
SFYDFEMQGFFI(4) SFYDEMGFI(4) SYFMQFF(3)	[2.6799±0.2464] [3.0158±0.2156] [3.1003±0.1193]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 34170090

Target Name:

Anti-infectious bronchitis virus (IBV) M41 strain S1 protein monoclonal antibody 3D9

Template Name:

Spike protein S1 of Spike glycoprotein

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The absorbance at 450 nm was measured using a Bio-Rad Microplate Reader.

Brief Description:

A standard biopanning procedure was carried out according to the manufacturer's instructions. Briefly, one well of a 96-well microtiter plate was coated with 100 µL of mAb 3D9 with a final concentration of 100 µg/mL. After blocking, 100 µL of the phages (about 2.0e11 pfu/mL) from the Ph.D.-12 phage library was added to the wells and incubated for 1 h at room temperature. The unbound phages were removed, and the wells were washed with 0.1%TBST. An elution buffer was added to each well with gentle shaking for 1 h at room temperature. The eluent containing the phages was mixed with a neutralization buffer to determine the titer of the phages and for amplification. The coated mAb 3D9 concentration (100 µg/mL, 75 µg/mL, and 50 µg/mL) was reduced and the concentration of Tween-20 in TBST buffer (0.1%, 0.2%, and 0.3%) was increased according to the number of biopanning steps.

BiopanningDataSet Comments:

Sequence analysis showed that the dominant sequence was SFYDFEMQGFFI. Indirect competitive enzyme-linked immunosorbent assay showed that SFYDFEMQGFFI is a mimotope of the S1 protein that was predicted by PepSurf. The mimotope may provide information for further structural and functional analyses of the S1 protein.

Curator:

Bowen Li

Curation Date:

2023-10-13

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