BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3565

Peptides:

Sequences
GWRVSEF(24) GWRVSEL(1) GFHYSLH(1) IVGSQVT(1)

Sequences Features:

Motif:

GWRVSE

Unique Sequence Number:

Experimental Method:

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 34314166

Target Name:

Cowpea mosaic virus, CPMV

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The plate was incubated in the dark for 10 min, and the absorbance was measured at 370 nm by using an Infinite 200 Rx plate reader (Tecan Life Sciences) with 25 flashes in 96-well flat-bottom plate mode.

Brief Description:

The input library was preincubated overnight with blocking agent (2% (w/v) bovine serum albumin; BSA) each round to remove potential BSA binders.

BiopanningDataSet Comments:

Biopanning and characterization of lead candidates resulted in isolation of the motif “GWRVSEF/L” as the plant virus cowpea mosaic virus (CPMV) specific motif with phenylalanine (F) at the seventh position being stronger than leucine (L). Specificity to CPMV was demonstrated, and cross-reactivity toward other plant viruses was not observed. To demonstrate cargo loading, GWRVSEF was tagged with biotin, fluorescein isothiocyanate (FITC), and a human epidermal growth factor receptor 2 (HER2)-specific targeting peptide ligand. Display of the active ingredient was confirmed, and utility of tagged and targeted CPMV in cell binding assays was demonstrated. The CPMV-binding peptides (CBP) functionalization strategy offers a new avenue for CPMV nanoparticle functionalization and should offer a versatile tool to add active ingredients that otherwise may be difficult to conjugate or display.

Curator:

Bowen Li

Curation Date:

2023-10-13

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