BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3563

Peptides:

Sequences

Affinity Values

HAMRAQP(8)
NAPDWPA(4)
SPSTHWK(3)
STSFWIT(2)
NESHSRT(2)
GFFHKTT(1)
LPAGRVL(1)
NGLTAWT(1)
AAPDWAG(1)
HAKRARA(1)
TLHPAAD(1)
HVQLWAT(1)
SAAIGTL(1)
LSNNNLR(1)

[243.3722±14.9784]
[87.7108±23.5104]
[24.0053±5.9724]
[41.7329±5.8776]
[26.1857±5.8776]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

HAXRA,APDW

Unique Sequence Number:

Experimental Method:

Sequencing Platform:

ABI automatic DNA analyzer (Applied BioSystems, USA)

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 35103339

Target Name:

Colon cancer cell line SW480

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Binding selectivity of the frequently occurring selected phage clones to colon cancer cells was analyzed by cell-based ELISA. ELISA values for binding of each phage clone to each cell type were calculated by dividing the OD of selected phage clone to the OD of control phage (no displayed peptide). Data are reproduced from Figure 4 and presented as the mean ± SD.

Brief Description:

In the first round of in vitro selection, an aliquot of the primary library was added to an empty well (depletion well). The supernatant composed of phages that do not bind to the plastic surface of six-well plates was removed and poured into a serum-treated well. The supernatant containing phages that do not interact with serum was removed and transferred to the well with blocked absorber cells. The subtracted phage supernatant was recovered, applied to the target SW480 cells.

BiopanningDataSet Comments:

According to the results of cell binding assay and phage cell-based ELISA, one of the isolated peptides denoted as CCBP1 (with the sequence HAMRAQP) was indicated to have the highest binding efficiency, selectivity, and specificity toward colon cancer cells with no significant binding to control cells. Peptide competitive inhibition assay revealed that binding of the phage-displayed CCBP1 is competitively inhibited by the same free peptide, suggesting that CCBP1 specific binding to the target cell is independent of the phage context. Taken together, our findings provide support for the notion that CCBP1 binds specifically to colon cancer cells and might be a potential lead candidate for targeted delivery of imaging agents or therapeutic genes/drugs to colon tumors.

Curator:

Bowen Li

Curation Date:

2023-10-13

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