BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3551

Peptides:

Sequences
SVSVGMKPSPRP

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 31812636

Target Name:

Recombinant envelope (E) protein TrX

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

After five rounds of bio-panning, phages showing high binding activity to the recombinant E protein based on an indirect enzyme-linked immunosorbent assay (ELISA) were selected (data not shown).

Brief Description:

The Ph.D.-12™ Phage Display Peptide Library (New England Bio labs, USA) was exposed to plates coated with purified recombinant protein Trx. Unbound phages were washed away, and specifically bound phages were eluted and amplified for a second set of screening. After five rounds of bio-panning, phages showing high binding activity to the recombinant E protein based on an indirect enzyme-linked immunosorbent assay (ELISA) were selected then sequenced.

BiopanningDataSet Comments:

Curator:

Chunchao Pu

Curation Date:

2020-12-29

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