BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3521

Peptides:

Sequences	Affinity Values
AHNHTPIKQKYL LTPHKHHKHLHA SAVQWFELNTHA	[7.41469] [23.9808] [5.38701]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 32375468

Target Name:

Pt–W Coils

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Binding assay

Affinity Measurement Description:

The stent or coil was observed by an upright fluorescence microscope (BX53-44-FL-1, Olympus, Tokyo, Japan).Fluorescence intensity of Pt−W coils surfaces after exposure to FITC-labeled peptides was determined. Fluorescence intensities were reproduced from Figure 3B in the reference and shown.

Brief Description:

To wash out nonbinding phages, the Pt–W coils were rinsed by PBS containing Tween 20 (0.1%) once and then by PBS four times at room temperature. The phages bound to the surface of the stent and coil were eluted with 37.5 μg/mL glycine/HCl solution (pH 2.2) containing 1 mg/mL BSA and 0.1 mg/mL phenol red for 15 min at room temperature. The eluted phage solution was neutralized with Tris-HCl (pH 9.1). Then, E. coli cells were infected with the eluted phages and cultured for the phage amplification. These amplified phages were used for the next round of panning. After the final panning at the fifth round, E. coli cells infected with the obtained phages were plated and incubated on LB-agar plates containing IPTG and X-gal (overnight at 37 °C).

BiopanningDataSet Comments:

Phage display method was used to identify peptide ligands that had high affinity for the metallic surface of Pt–W coils. The binding assay using fluorescence labeling revealed that several synthetic peptides could bind onto those surfaces.

Curator:

Chunchao Pu

Curation Date:

2020-12-29

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