BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3516

Peptides:

Sequences	Affinity Values
NISAWFQPHQAL(13) WQWGPYMVGAGV(4)	[0.270370±0.088490] [NA]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3-5

Reference:

PMID: 32717427

Target Name:

Multiple Sclerosis recombinant antibodie A2, MS-A2 (ST-196)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 and Ph.D.-7 phage library pool

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Absorbance at 405 nm was determined spectrophotometrically with a Microplate Reader (Bio-Rad, Hercules, CA). Data (OD405) shown were reproduced from Figure 1B in the reference.

Brief Description:

The Phage Display Peptide Libraries (12mer + 7mer) (New England BioLabs, Beverly, MA) were used for affinity selection of specific peptides under five rounds of biopannning for the MS brain rAbs.

BiopanningDataSet Comments:

We identified 17 high affinity phage peptides from which 2 peptides(NISAWFQPHQAL,WQWGPYMVGAGV) are unique. Database searches revealed that peptide A2-D2(WQWGPYMVGAGV) shared sequence homologies with G-protein coupled receptor 161,Myomesin-3 and RNA-directed RNA polymerase L [Oliveros mammarenavirus]. And peptide A2-F4(NISAWFQPHQAL) shared sequence homologies with Dual specificity protein phosphatase 2,Ubiquitin-like modifier-activating enzyme Epstein-Barr nuclear antigen 6 and Capsid assembly protein [Human herpesvirus 4 strain B95-8].

Curator:

Chunchao Pu

Curation Date:

2020-12-29

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