BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3511

Peptides:

Sequences	Affinity Values
SCATPFSPQVCS(78/80) SCRLQVXPGFCS(1/80) SCGERGXAECCS(1/80)	[0.30566] [NA] [NA]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Genetic Analyzer 3130

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 31422560

Target Name:

Copper (II) oxide nanoparticles, CuO-NPs

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

T7 SCX8CS phage display library (SCX8CS)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The absorbance of each sample was measured at 450 nm using a microplate reader (Spectra Max Plus 384; Molecular Devices, San Jose, CA, USA). Data (OD450) shown were reproduced from Figure 1b in the reference.

Brief Description:

Prepared CuO-NPs (500 μg) were mixed with the constructed libraries and incubated in TBS-T containing 0.1% Tween 20. After 1 h at room temperature, CuO-NPs were washed five times with TBS-T. For the proliferation of T7 phages bound to the surface of CuO-NPs, E. coli BLT5403 (Merck Millipore) proliferated to the log phase was mixed with the nanoparticles and incubated at 37 °C.

BiopanningDataSet Comments:

We evaluated the cytotoxicity of copper (II) oxide nanoparticles (CuO-NPs) by coating with a novel cyclic peptide, CuO binding peptide 1 (CuBP1), cyclic-SCATPFSPQVCS, which binds to the surface of CuO-NPs. CuBP1 was found to promote the aggregation of CuO-NPs under mild conditions. The treated CuO-NPs with CuBP1 caused the reduction of the cytotoxicity against Escherichia coli, Lactobacillus helveticus, and five other microorganisms, including bacteria and eukaryotes. Similar effects were also demonstrated against human embryonic kidney (HEK293) cells in vitro.

Curator:

Chunchao Pu

Curation Date:

2020-12-29

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