BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3509

Peptides:

Sequences
DWSSWVYRDPQT(10%) SGVYKVAYDWQH(5%)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 31902944

Target Name:

Folate receptor alpha

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Bound phages were eluted by addition of 1 mL of folic acid (Acros organics, Fisher Scientific, UK) in PBS (100 µg/mL) by competitive inhibition for 1 h at 4 °C.

BiopanningDataSet Comments:

Two phage peptides(DWSSWVYRDPQT,SGVYKVAYDWQH) showed binding specificity to SKOV3 cells in vitro screened by Ph.D.-12 phage display library. WSSWVYRDPQT peptide identified targets colon cancer cells in vitro with in silico analysis suggesting the peptide targets glypican-3 (a heparin sulphate proteoglycan (HSPG)).

Curator:

Chunchao Pu

Curation Date:

2020-12-29

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