BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3491

Peptides:

Sequences

Affinity Values

CHMRQGMAC(33/50)
CSYPDTHRC(2/50)
CQHPGSTMC(2/50)
CTATESTVC(2/50)
CNDWLSNSC(2/50)
CQQGLSSQC(1/50)
CHGPRASQC(1/50)
CKQTTSSSC(1/50)
CPLAYPHTC(1/50)
CKETWWPHC(1/50)
CMHTQTPWC(1/50)
CTTHTYFGC(1/50)
CGLKHTLKC(1/50)
CLARNATWC(1/50)

[1.40139]
[0.3324]
[0.31154]
[0.38348]
[0.49799]
[0.79257]
[0.39923]
[0.78065]
[0.77129]
[1.19017]
[0.34602]
[0.63293]
[0.48309]
[0.50906]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 31247207

Target Name:

Plasma from 10 Alzheimer's disease patients

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The absorbance value at 450 nm was determined with a microplate reader. Data (OD450) shown were reproduced from Figure 2C in the reference.

Brief Description:

In the first round of selection, phage library was selected against the pooled Alzheimer's disease (AD) plasma with albumin/IgG depletion. The second round was negative selection against pooled plasma from 10 Controls (5 male and 5 female) without albumin/IgG depletion. The third and fourth rounds of selection were against the same pooled AD plasma with albumin/IgG depletion.

BiopanningDataSet Comments:

We further characterized one AD-specific peptide (AD#1 peptide, CHMRQGMAC) and one control-specific peptide (Con#1 peptide, CDGARHGRC), and evaluated their diagnostic performance in independent validation set (35 AD patients, 45 MCI, 45 controls and 20 PD patients). Our results show that both AD#1 peptide and Con#1 peptide could distinguish AD/MCI patients from controls and combition of these two peptides could greatly improve the diagnostic performance (AUC is above 0.80 in ROC curve alysis). In addition, we found that AD#1 peptide stained Aβ-treated primary astrocyte and bound to recombint human YKL-40 protein in in-vitro assay. It supports that AD#1 peptide detects AD inflammation related cytokine.

Curator:

Chunchao Pu

Curation Date:

2020-12-01

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