BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3488

Peptides:

Sequences
CSLTQYRYC(1/15)

Sequences Features:

Motif:

XTQ

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 31778678

Target Name:

Anti-D-antigen monoclonal antibody 1G10

Template Name:

D-antigen

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Three phage clones were selected because of their high frequency in bio-panning, good affinity and specificity for binding to mAb 1G12 in phage ELISA , as well as their similarity with the epitope of PV-I Sabin antigen performed in phage competitive ELISA_x0000_

Brief Description:

Phage clones were selected by solution-phase panning with protein G magnetic beads (NEB) that exhibited high affinity for IgG.

BiopanningDataSet Comments:

Curator:

Chunchao Pu

Curation Date:

2020-12-01

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