BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3482

Peptides:

Sequences
*CSLTQDQGC(328,760) CADAQADVC(140,578) CKASRLGRC(139,938) CRAKGRDAC(103,044) CVGRPDAC(84,760) CSRTEGDVC(67,529) CSSLVKTC(61,014) CKRSSYFC(57,309) CRNGEGAQC(54,832) CGELCNDC(54,226)*

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Sequencing Platform:

Illumi

Sequencing Method:

Next-generation sequencing

Rounds of Panning:

Reference:

PMID: 32035108

Target Name:

Inflamed central nervous system (CNS) of mice with experimental autoimmune encephalomyelitis (EAE)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX7C T7 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

A tissue homogete equivalent to 100 mg of tissue was incubated with phage library at 4 °C for 1 h. Thereafter, the tissue homogete was washed to remove the unbound phages, whereas the bound-phages were rescued using BLT5615 strain of E. coli. The phages were then amplified and phages were injected intravenously (i.v.) into EAE mice that had clinical score between 2 and 3. The phages were circulated for 15 min. Thereafter, animals were anesthetized, perfused with saline, and CNS tissue was collected and homogenized.

BiopanningDataSet Comments:

MS-1 (amino acid sequence CRGGKRSSC), was shown to selectively recognize CNS tissue in EAE mice. Individually cloned phages with this insert preferentially homed to EAE CNS after an intravenous injection. Similarly, systemically-administered fluorescence-labeled synthetic MS-1 peptide showed selective accumulation in the spil cord of EAE mice.

Curator:

Chunchao Pu

Curation Date:

2020-12-01

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