BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3475

Peptides:

Sequences
CRPTYSPSC CKIHSSETC CPKSNNGVC CIGNSNTLC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30850705

Target Name:

Human colorectal cancer cell line RKO

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

An adaptation of the BRASIL method using RKO cells as target was used. A total of four rounds of selection with human epithelial CRC cell line RKO (ATCC CRL 2577) were performed through in vitro biopanning. In each round, the phages that specifically bound to target cells were recovered and used for the next round of selection. In the three initial rounds of selection, the obtained phage pool was not amplified between rounds.

BiopanningDataSet Comments:

Curator:

Pengcheng Yao

Curation Date:

2019-08-17

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