BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3470

Peptides:

Sequences

Affinity Values

RCLVPCRYLCW
RCLLPCRYWCE
RCVRPCYFPCA
GCSWWCNRACP
VCLMICHYPCL
RCLVPCRLHCW
RCLLPCRYWCP
RCLVPCKYWCY
RCLLPCNLWCF
RCLGPCWIVCP
RCVRPCFIPCA
RCVRPCPWTCS
RCVAPCWWGCN
RCQYWCNRACL
TCSWWCNRSCL
GCSWWCNRACL
LCYQICHYPCS
TCFFICHYPCL

[>200]
[>200]
[>200]
[>200]
[>200]
[NA]
[NA]
[NA]
[NA]
[NA]
[NA]
[NA]
[NA]
[NA]
[NA]
[NA]
[NA]
[NA]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

2/3

Reference:

PMID: 24453110

Target Name:

Urokinase-type plasminogen activator

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

XCX3CX3CX phage display library

Affinity Measurement Method:

Inhibition assay

Affinity Measurement Description:

The inhibition constants Ki (μM) were determined by incubating different concentrations of bicyclic peptide with fixed concentration of human urokinase-type plasminogen activator. The indicated Ki values are averages of at least two measurements.

Brief Description:

The peptide library was cyclized with the hydrophilic small molecule 1,3,5-triacryloyl-1,3,5-triazinane (TATA). The library was then subjected to two or three iterative rounds of phage selection against human urokinase-type plasminogen activator.

BiopanningDataSet Comments:

Curator:

Bifang He

Curation Date:

2019-08-17

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