BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3458

Peptides:

Sequences	Affinity Values
LPSYPGYYQITI(7) CWTISLFGGVTQ(3) SKRSTGKDYTAT(1) RRVISARWTSDR(1) MQTSFLKHHSVT(1)	[1.860 ± 0.056] [1.576 ± 0.055] [1.461 ± 0.078] [1.461 ± 0.055] [1.306 ± 0.066]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 31149727

Target Name:

Apical membrane antigen-1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The optical density (OD) was measured at 490 nm using a microplate reader. The OD values were reproduced from Figure 2 and shown.

Brief Description:

BiopanningDataSet Comments:

Two corresponding specific Eimeria tenella apical membrane antigen-1 (EtAMA1) binding peptides (LPSYPGYYQITI (L) and CWTISLFGGVTQ (C)) showed significant effects on inhibiting sporozoite invasion of MDBK cells.

Curator:

Lixu Jiang

Curation Date:

2019-08-17

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