BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3449

Peptides:

Sequences

VGSYSATVHRIQ
VLAHKYTTGSLP
ACLYCYDLVDSV
SLPLTVVPTANR
TLGLRPVPVATT
TNSFHGIAGYQS
SSLLGYNLNANH
ASHTGTAQISRQ
ETLKPYPLPNNS
DGMLSLSLPASF
VRATQYPMLLDF
NGPSDLKYLHPT
HPHDRQLISTYP
NDVGVLSPETRQ
IEQRHQTHSLHP
HMSYGNGTRDTP
DNHAKLPLRGSS
VVFDGTLYNYHW
DIARTAKPSHWP
AYTVDALHELRH
SYEPNSTKQFAP
VDDLVRVSVNFR
MYNPDPNRLARS
WHYNWQDVSDRQ
NMGGLDYLNKNS
EALTVNIKREME
HLTATELANSYH
YTLSDRSHNPFN
SWIPWAAFEHKQ
WTTAAPAKSEYH
TPVQQGQTKNAF
FMIPGSMPYIGC
CLYCWQGTSQQR
WDWPLFPLDTLR
AYEPSSHKSEVP
VPIHAAHGFWHY
WPAPFHNLVNPR

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30187588

Target Name:

IgE from patients with allergy to Scylla paramamosain SCP

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Overnight, coating of the wells of microtiter plate (Nunc Maxisorp, Copenhagen, Denmark) at 4°C with goat anti-human IgE (ε‐chain specific) (Sigma‐Aldrich, St. Louis, MO, USA) was used to capture IgE from patients’ sera. The phage display peptide library was added after the blocking incubation with captured IgE and eluted after the washing steps.

BiopanningDataSet Comments:

Curator:

Bifang He

Curation Date:

2019-05-28

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