BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 3431

Peptides:

Sequences	Affinity Values
ACPMNESKFC(12/22) ACPSNPSKFC(6/22) ACPKNPNKFC(2/22)	[29.1] [32.1] [31.4]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 30262856

Target Name:

Natalizumab

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Biolayer interferometry (BLI)

Affinity Measurement Description:

To determine binding constants for association and dissociation of natalizumab with Ntz-01–07, biolayer interferometry (BLI) was performed by Antibody Solutions (Mountain View CA), using an Octet Red96 (Pall ForteBio LLC, Fremont CA). Briefly, 10 μg/ml of peptide was immobilized onto a streptavidin sensor. Three-fold dilutions of natalizumab were in solution, at concentrations from 1–600 nM (7 total dilutions). The assay steps were: sensor check (30 s), load antibody (600 s), baseline (300 s), antibody association (550 s), antibody dissociation (550 s). TBS was used as the buffer. KD (nM) for synthesized peptides was determined and shown.

Brief Description:

BiopanningDataSet Comments:

PKNPSKF is identified as a novel natalizumab-binding motif, and peptides containing this motif demonstrated utility as capture reagents in enzyme-linked immunosorbent assays (ELISAs). A peptide containing the identified motif was shown to be competitive with the natural ligand (α4-integrin) and a neutralizing anti-idiotype antibody for natalizumab binding.

Curator:

Bifang He

Curation Date:

2019-05-28

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